University of Cambridge > > Morphogenesis Seminar Series > CADHERIN mediated AMIS localisation - Using mouse embryonic stem cells 3D culture as a model

CADHERIN mediated AMIS localisation - Using mouse embryonic stem cells 3D culture as a model

Add to your list(s) Download to your calendar using vCal

  • UserXuan Liang (PDN, Cambridge University)
  • ClockMonday 31 January 2022, 14:30-15:30
  • HouseOnline.

If you have a question about this talk, please contact Elena Scarpa.

Join the mailing list for the zoom link

ndividual cells within de novo polarising tubes and cavities must integrate their forming apical domains into a centralised apical membrane initiation site (AMIS). This is necessary to enable organised lumen formation within multi-cellular tissue. Despite the well documented importance of cell division in localising the AMIS , we have found a division-independent mechanism of AMIS localisation that relies instead on CADHERIN -mediated cell-cell adhesion. Our study of de novo polarising mouse embryonic stem cells (mESCs) cultured in 3D suggest that cell-cell adhesion directs the localisation of apical proteins such as PAR -6 to a centralised AMIS . Unexpectedly, we also found that mESC cell clusters lacking functional E-CADHERIN were still able to form a lumen-like cavity in the absence of AMIS localisation and did so at a later stage of development via a ‘closure’ mechanism, instead of via hollowing. This work suggests that there are two, interrelated mechanisms of apical polarity localisation: cell adhesion and cell division. Alignment of these mechanisms in space allows for redundancy in the system and ensures the development of a coherent epithelial structure within a growing organ.

This talk is part of the Morphogenesis Seminar Series series.

Tell a friend about this talk:

This talk is included in these lists:

Note that ex-directory lists are not shown.


© 2006-2024, University of Cambridge. Contact Us | Help and Documentation | Privacy and Publicity