Visualization of low-level Gene Expression and Biomarker Localization within Tissue: Applications of RNAscope® and BaseScope

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• Gemma Hughes; Advanced Cell Diagnostics
• Wednesday 19 September 2018, 13:00-13:30
• Babraham - The Cambridge Building; Kings Hedges Room.

If you have a question about this talk, please contact Bobbie Claxton.

If you would like to attend this seminar, please contact us to arrange site access.

Spatially mapped biomarker information at the single-cell level is crucial to understanding cellular organization and cell-to-cell interactions in complex tissue environment, and understanding cellular interactions within gene and cell therapy models. RNAscope® and BaseScope™ assays, the two highly specific and sensitive ISH methods allow visualizing gene expression and genetic variations in tissue environments. This seminar will highlight: • Detection of mRNAs, viral RNAs, and lncRNAs at single molecule sensitivity by RNAscope® assay • Use as cell markers for cell therapy T-cell and viral vector localization as a sensitive safety assessment tool • Validation of target gene expression after high-throughput transcriptome analysis • Target specific splice junctions by BaseScope™ assay • Distinguish specific homologues, paralogues, and orthologues by RNAscope® or BaseScope™ assays • Perform single cell analysis within tissue architecture in FFPE or frozen tissues

This talk is part of the Babraham Seminar series.

This talk is included in these lists:

• Babraham - The Cambridge Building; Kings Hedges Room
• Babraham Seminar
• Graduate-Seminars
• se393's list

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