Single cell seminar: "Duplex sequencing at genome scale"; "Charting the diversification of mammalian cells at whole organism scale"

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• Rob Osborne (CASM, Sanger); Jonny Griffiths (CRUK CI)
• Friday 27 April 2018, 15:00-16:00
• C302, Sulston Building, Wellcome Genome Campus, Hinxton. CB10 1SA.

If you have a question about this talk, please contact Lia Chappell.

All are welcome, including colleagues from Cambridge (though email organiser so we can book you in as a visitor with security)

Please come along the monthly campus Single Cell Seminar series. All are welcome, including colleagues from Cambridge (though email us so we can book you in as a visitor with security)

This month we have two exciting talks, please join us C302 from 3.00 – 4.00pm THIS FRIDAY (27th April)

1) Rob Osborne (CASM, Sanger): “Duplex sequencing at genome scale”

2) Jonny Griffiths (CRUK CI): “Charting the diversification of mammalian cells at whole organism scale”

Rob’s abstract: “High-throughput sequencing of somatic mutations is required for clinical medicine and for fundamental questions in cancer, aging and normal development. Current sequencing methods trade off analytical specificity with genome-coverage. Bulk sequencing has low specificity but high genome-coverage. In contrast, sequencing methods incorporating error-correction have high specificity but are inefficient and expensive to apply to whole-genomes. I will describe progress towards an improved duplex sequencing method that provides a simple and automatable route to accurate genome-wide detection of somatic mutations.”

Jonny’s abstract: “Decision-making is a critical component of cellular behaviour, with errors giving rise to e.g., developmental failures or cancer. Embryonic development is an effective system for studying decision-making, as it provides a large set of robust, biologically relevant, relatively well-studied decision points. However, transcriptomic assays have historically been hamstrung by the extreme spatial complexity and molecular heterogeneity of the developing embryo.

We have captured 100,000 single cells for single-cell RNAseq from whole mouse embryos during gastrulation and organogenesis, spanning days 6.5 to 8.5 of development, including embryonic and extraembryonic tissues. Cells were sampled every six hours, providing a continuous molecular characterisation of these processes. We highlight the utility of this rich dataset in three ways. First, we reconstruct the development of the three germ layers and their lineages. Second, we identify non-negligible populations of rare cell types such as primordial germ cells. Third, we dissect one specific developmental process by considering the contribution of extraembryonic visceral endoderm to embryonic endoderm lineages.”

This talk is part of the Single Cell seminars at the Wellcome Genome Campus series.

This talk is included in these lists:

• C302, Sulston Building, Wellcome Genome Campus, Hinxton. CB10 1SA
• Single Cell seminars at the Wellcome Genome Campus

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