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SUMMARY:Lighting up RNA Interactions in Living Cells - David Tollervey\, W
 TCCB\, University of Edinburgh
DTSTART:20140210T140000Z
DTEND:20140210T150000Z
UID:TALK48299@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:In vivo analyses of RNA processing are impeded by two major pr
 oblems: 1) The kinetics of the process must generally be inferred from ste
 ady-state levels of intermediates\, so rapid processing events are essenti
 ally invisible\, particularly if they occur cotranscriptionally. 2) The st
 ructures of RNA-protein complexes\, within which almost all RNA processing
  and RNP assembly steps occur\, generally remain poorly characterized. The
 se limitations are particularly acute for low abundance members of complex
  populations\, such as pre-mRNAs or ncRNAs. We have therefore been priorit
 izing techniques to overcome these limitations. Fast kinetic labeling supp
 orted by mathematical modeling to analyses rapid processing. UV crosslinki
 ng (CRAC) to determine sites of RNA-protein interaction. Crosslinking\, li
 gation and sequencing of hybrids (CLASH) to experimentally identify RNA-RN
 A interactions. These techniques were developed to understand fundamental 
 RNA metabolism in budding yeast – but are being applied in systems from 
 pathogenic E.coli to virus-infected mammalian cells. 
LOCATION:Max Perutz Lecture Theatre\, Medical Research Council (MRC) (MRC 
 Laboratory of Molecular Biol
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