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SUMMARY:LINE-1 retrotransposition in stem cells - Dr Jose Luis García Pé
 rez\, Universidad de Granada\, Junta de Andalucía Centre for Genomics and
  Oncological Research
DTSTART:20120509T153000Z
DTEND:20120509T163000Z
UID:TALK37730@talks.cam.ac.uk
CONTACT:Suzy Blows
DESCRIPTION:Long INterspersed Element class 1 (LINE-1 or L1) retrotranspos
 ons comprise a fifth of the human genome and their ongoing activity contin
 ues to impact the genome. Heritable LINE-1 retrotransposition events must 
 occur in cell types that ensure their transmission to newborns. Previous r
 eports have detected LINE-1 expression and activity of engineered elements
  in cultured human cells that mimic an early embryo. These include human e
 mbryonic stem cells (hESCs)\, human embryonic carcinoma cells (hECs) and i
 nduced pluripotent stem cells (iPSCs). Additionally\, recent reports have 
 revealed the expression and mobilization of LINE-1 elements in selected so
 matic human cells including Neuronal Progenitor Cells (NPCs). Here\, we us
 ed a panel of pluripotent and differentiated cell cultures to inspect the 
 expression and retrotransposition levels of engineered LINE-1 retrotranspo
 sons. Remarkably\, we observed that LINE-1s are expressed in pluripotent n
 euronal and embryonic cell types\, although expression is higher in embryo
 nic cell types. Additionally\, we recapitulated engineered LINE-1 retrotra
 nsposition in pluripotent neuronal and embryonic cell types\, but observed
  that retrotransposition rates can vary more than 200-fold among different
  pluripotent cell types. Indeed\, under our experimental conditions\, the 
 highest rate of engineered LINE-1 retrotransposition is observed in neuron
 al cell types. These data suggest that the main load of LINE-1 retrotransp
 osition in humans may occur in selected somatic cells and that most mobili
 zation events will not be transmitted to newborns.
LOCATION:Lecture Theatre 1\, Department of Veterinary Medicine
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