BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Dehydrated but unharmed? – the mass spectrometry of protein comp
 lexes - Professor Carol Robinson\, Department of Chemistry\,  Oxford Unive
 rsity
DTSTART:20101122T161500Z
DTEND:20101122T180000Z
UID:TALK27522@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:Polydispersity\, heterogeneity and insolubility are among the 
 most challenging properties of protein complexes making them almost imposs
 ible to study by conventional structural biology approaches.  Building on 
 our early experiments of intact ribosomes we have recently explored the su
 bunit architecture of the eukaryotic initiation factor 3 (eIF3) and the ro
 le of post translational modifications in ribosomal proteins and their com
 plexes. \nOur recent discoveries that large integral membrane protein comp
 lexes can survive intact in the mass spectrometer (Barrera\, Di Bartolo et
  al. 2008\; Barrera\, Isaacson et al. 2009) allow us to apply mass spectro
 metry methods to some of the most challenging and controversial membrane p
 rotein complexes studied to date.  \nSpecifically I will describe how the 
 lipid binding properties within these protein complexes is enabling us to 
 shed new light on their structure and function. I will also describe how i
 on mobility measurements are constraining molecular models of membrane sub
 units within the intact ensemble of structures.  \nReferences \n\nBarrera\
 , N. P.\, N. Di Bartolo\, et al. (2008). "Micelles protect membrane comple
 xes from solution to vacuum." Science 321(5886): 243-246.\nBarrera\, N. P.
 \, S. C. Isaacson\, et al. (2009). "Mass spectrometry of membrane transpor
 ters reveals subunit stoichiometry and interactions." Nature Methods 6(8):
  585-587.\n\n\n
LOCATION:Max Perutz Lecture Theatre\, Medical Research Council (MRC) (MRC 
 Laboratory of Molecular Biol
END:VEVENT
END:VCALENDAR