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SUMMARY:Fluorescence-Lifetime Super-Resolution Microscopy - Prof. Dr. Jör
 g Enderlein\, Institute of Physics – Biophysics\, Georg August Universit
 y\, Germany
DTSTART:20260224T140000Z
DTEND:20260224T150000Z
UID:TALK243379@talks.cam.ac.uk
CONTACT:Leia Pryke
DESCRIPTION:Recent advancements in super-resolution microscopy have enable
 d unprecedented insights into the spatial organization of cellular structu
 res. In this talk\, I will present a series of methodological innovations 
 that synergistically integrate fluorescence-lifetime single-molecule local
 ization microscopy (FL-SMLM)  [1\,2]\, image scanning microscopy (ISM)  [3
 \,4]\, and metal-/graphene-induced energy transfer (MIET/GIET) imaging  [5
 –7]. These approaches collectively offer isotropic three-dimensional res
 olution at the nanometer scale\, multiplexed imaging capabilities\, and ro
 bustness against chromatic aberrations.\nFirst\, I will discuss our work o
 n MIET and GIET microscopy\, which exploit distance-dependent quenching ph
 enomena near metallic or graphene interfaces to determine the axial positi
 on of single emitters with sub-10 nm accuracy. The combination of MIET wit
 h dSTORM or DNA-PAINT provides truly isotropic 3D resolution\, extending t
 he reach of localization microscopy into the axial dimension without inter
 ferometric complexity.\nSecond\, I will highlight the development of fluor
 escence lifetime DNA-PAINT (FL-PAINT)\, a technique that enables multi-tar
 get super-resolution imaging through fluorescence lifetime multiplexing wi
 thout fluid exchange. By utilizing orthogonally designed imager strands co
 njugated to fluorophores with distinct lifetimes\, we achieve simultaneous
  imaging of multiple targets in the dense intracellular environment.\nLast
 ly\, I will introduce our latest development of fluorescence-lifetime imag
 e scanning microscopy SMLM (FL-iSMLM)\, which achieves a near twofold enha
 ncement in lateral resolution by integrating a single-photon detector arra
 y into a confocal laser scanning microscope. This method combines the loca
 lization precision of ISM with the multiplexing power of fluorescence-life
 time detection\, enabling sub-5 nm resolution in fixed cells while simulta
 neously allowing discrimination of targets based solely on their fluoresce
 nce lifetimes.\nReferences\n[1]	J. C. Thiele\, D. A. Helmerich\, N. Oleksi
 ievets\, R. Tsukanov\, E. Butkevich\, M. Sauer\, O. Nevskyi\, and J. Ender
 lein\, Confocal Fluorescence-Lifetime Single-Molecule Localization Microsc
 opy\, ACS Nano 14\, 14190 (2020).\n[2]	J. C. Thiele\, O. Nevskyi\, D. A. H
 elmerich\, M. Sauer\, and J. Enderlein\, Advanced Data Analysis for Fluore
 scence-Lifetime Single-Molecule Localization Microscopy\, Front. Bioinform
 . 1\, 740281 (2021).\n[3]	C. B. Müller and J. Enderlein\, Image Scanning 
 Microscopy\, Phys. Rev. Lett. 104\, 198101 (2010).\n[4]	N. Radmacher\, O. 
 Nevskyi\, J. I. Gallea\, J. C. Thiele\, I. Gregor\, S. O. Rizzoli\, and J.
  Enderlein\, Doubling the resolution of fluorescence-lifetime single-molec
 ule localization microscopy with image scanning microscopy\, Nat. Photon. 
 18\, 1059 (2024).\n[5]	A. I. Chizhik\, J. Rother\, I. Gregor\, A. Janshoff
 \, and J. Enderlein\, Metal-induced energy transfer for live cell nanoscop
 y\, Nature Photon 8\, 124 (2014).\n[6]	A. Ghosh\, A. Sharma\, A. I. Chizhi
 k\, S. Isbaner\, D. Ruhlandt\, R. Tsukanov\, I. Gregor\, N. Karedla\, and 
 J. Enderlein\, Graphene-based metal-induced energy transfer for sub-nanome
 tre optical localization\, Nat. Photonics 13\, 860 (2019).\n[7]	J. C. Thie
 le\, M. Jungblut\, D. A. Helmerich\, R. Tsukanov\, A. Chizhik\, A. I. Chiz
 hik\, M. Schnermann\, M. Sauer\, O. Nevskyi\, and J. Enderlein\, Isotropic
  Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induc
 ed Energy Transfer\, Science Advances 8\, 14190 (2021).\n\n
LOCATION:Yusuf Hamied Department of Chemistry\, Unilever Lecture Theatre
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