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SUMMARY:Expanding BioID: the nuclear pore complex of trypanosomes - Dr Sus
 anne Kramer - Universität Wüerzburg - Germany.
DTSTART:20240306T140000Z
DTEND:20240306T150000Z
UID:TALK207328@talks.cam.ac.uk
CONTACT:Anna Protasio
DESCRIPTION:Proximity labelling by a biotin ligase combined with mass spec
 trometry of biotin-affinity purified proteins (BioID) has become a powerfu
 l tool to investigate protein interactions in vivo in a range of organisms
 \, including trypanosomes. We recently discovered an interesting off-label
  application of BioID for protein imaging by fluorescent streptavidin. We 
 found streptavidin imaging superior to classical antibody labelling becaus
 e it (i) provides a stronger signal with no loss in resolution (ii) can im
 age proteins in phase-separated regions that are not accessible to antibod
 ies and (iii) provides information on localization dynamics\, since “his
 toric” interactions are preserved.\nWe have used the method\, in combina
 tion with expansion microscopy\, classical BioID\, neural-network based in
  silico predictions and reverse genetics to reinvestigate the trypanosome 
 nuclear pore complex.\n
LOCATION:Seminar Room\, Tennis Court Road\, Dept of Pathology.
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