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CATEGORIES:Spring School 2009 - &quot\;Regeneration and Plast
 icity of Neural Circuits&quot\;
SUMMARY:In vivo 2-photon imaging of axonal microlesions in
  the adult brain - Vincenzo De Paola MRC Clinical 
 Sciences Centre\, Faculty of Medicine\, Imperial C
 ollege London
DTSTART;TZID=Europe/London:20090402T110000
DTEND;TZID=Europe/London:20090402T114500
UID:TALK17662AThttp://talks.cam.ac.uk
URL:http://talks.cam.ac.uk/talk/index/17662
DESCRIPTION:\nDysregulation of synaptic structure and function
  forms the basis for many neurological disorders c
 reating an urgent need to determine the mechanisms
  underlying functional reorganisation of the CNS. 
 Coupled with synaptic decline\, degeneration of th
 e axonal compartment\, as opposed to cell death\, 
 often precedes or is associated with the appearanc
 e of symptoms of disease. Understanding the mechan
 isms of axonal response to injury therefore become
 s imperative in detecting and understanding the ea
 rly stages of disease. Major efforts in this field
  have so far concentrated on spinal cord injury mo
 dels. To gain a comprehensive view of axonal respo
 nses to injury in the adult CNS\, we induced discr
 ete axonal lesions in the intact brain. By combini
 ng neuron-specific green fluorescent protein trans
 genic mice and in vivo 2-Photon microscopy through
  a cranial window we monitored the temporal dynami
 cs of reorganisation of the surviving neuron and t
 he degeneration of the disconnected axon. We find 
 that lesions can be targeted to identified axons. 
 Most disconnected axons (75%) degenerate rapidly w
 ithin 24h\, with the remaining 25% degenerating wi
 thin 48h. Degeneration of the proximal part is loc
 al\, comprising 30-400 microns from the lesion sit
 e. There is no attempt of growth from the proximal
  end up to three weeks post-lesion. We are in the 
 process of quantifying 1) synaptic remodelling in 
 the proximal side and 2) the induced damage in ter
 ms of cell death\, sub-cellular damage\, glia resp
 onses. \n\n
LOCATION:Cripps Court\, Magdalene College
CONTACT:Anna Di Pietro
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