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CATEGORIES:Babraham Seminar
SUMMARY:Chromatin and Gene Transcription in Hypoxia - Prof
 . Sonia Rocha\; Executive Dean of the Institute of
  Systems\, Molecular and Integrative Biology\, Uni
 versity of Liverpool
DTSTART;TZID=Europe/London:20210617T123000
DTEND;TZID=Europe/London:20210617T133000
UID:TALK157786AThttp://talks.cam.ac.uk
URL:http://talks.cam.ac.uk/talk/index/157786
DESCRIPTION:Oxygen is essential for the life of most multicell
 ular organism and changes in oxygen availability h
 ave implications in physiological and pathophysiol
 ogical conditions. In response to hypoxia (low oxy
 gen availability) transcriptional changes are indu
 ced. The transcriptional response to hypoxia is me
 diated primarily by the Hypoxia Inducible Factor (
 HIF) family of transcription factors. HIFs are act
 ivated in hypoxia via inhibition of prolyl hydroxy
 lases (PHDs)\, a group of molecular dioxygenases\,
  which require oxygen for their activity. Whilst t
 he role of HIFs in the transcriptional response to
  hypoxia is well characterised\, little is known w
 ith regards to chromatin structure in low oxygen s
 tress and how this coordinates the transcriptional
  response. It was recently shown that chromatin is
  sensitive to oxygen levels\, with hypoxia alterin
 g histone methylation at hypoxia responsive genes 
 via inhibition of specific JmjC histone demethylas
 es. To further investigate chromatin structure and
  its role in hypoxia induced gene transcription\, 
 we have performed ATAC-seq and RNA-seq in human ca
 ncer cell lines in acute and prolonged hypoxia. We
  find that acute hypoxia leads to increased promot
 er accessibility at a subset of hypoxia inducible 
 genes\, and this mostly precedes changes in mRNA e
 xpression\, with further changes in chromatin stru
 cture after prolonged hypoxia. In addition\, ChIP-
 seq data on histone methylation marks (H3K4me and 
 H3K36me3)\, revealed similar earlier patterns of g
 enomic distribution. Furthermore\, we dissected th
 e impact of HIF in the observed changes of ATAC-se
 q analysis. This work gives insights into the role
  of chromatin dynamics in regulating the transcrip
 tional response to hypoxia and elucidating the mec
 hanisms behind these changes may lead to targeted 
 therapies for hypoxia driven diseases.\n\nJoin the
  webinar live using this link – https://zoom.us/j/
 93010032634
LOCATION:Online via zoom
CONTACT:Bobbie Claxton
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