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CATEGORIES:Biological Chemistry Research Interest Group
SUMMARY:Fluorescent Nucleoside Analogues with New Properti
 es for Biophysics - Byron W Purse San Diego State 
 University
DTSTART;TZID=Europe/London:20191106T160000
DTEND;TZID=Europe/London:20191106T170000
UID:TALK134293AThttp://talks.cam.ac.uk
URL:http://talks.cam.ac.uk/talk/index/134293
DESCRIPTION:Biophysical probes for the study of nucleic acids 
 are essential tools in the endeavor to understand 
 the regulation and expression of the genetic code.
  While many capable fluorescent nucleoside analogu
 es exist for these applications\, it has been espe
 cially challenging to design analogues that mainta
 in Watson–Crick hydrogen bonding\, offer fluoresce
 nce brightness similar to conventional probes\, an
 d that absorb and emit at long wavelengths. Moreov
 er\, it is not yet possible to predict how the flu
 orescence of nucleobase analogues will respond to 
 base pairing and stacking. Towards our goal of dev
 eloping nucleoside analogues with new and enhanced
  fluorescent properties and plugging knowledge gap
 s in the relationship between base analogue struct
 ure and photophysics\, we have designed a series o
 f cytidine analogues with a range of structural mo
 difications and studied their fluorescent response
 s to base pairing and stacking and the efficiency 
 of their incorporation by DNA and RNA polymerases.
  This series of cytidine analogues shows clear rel
 ationships between analogue electronic properties\
 , the electronics of neighboring bases\, and the f
 luorescent responses to base pairing and stacking\
 , including with mismatches. One of the analogues\
 , DEA-tC\, offers a powerful\, sequence-specific f
 luorescence turn-on response to base pairing and s
 tacking that is further enhanced in DNA/RNA hetero
 duplexes. The combination of photophysical studies
 \, NMR structure determination of representative d
 uplexes\, and computational work helps to explain 
 the observed photophysical properties and relate t
 hem back to cytidine analogue structure and neighb
 oring base effects.
LOCATION:Pfizer  Lecture Theatre\,  Department of Chemistry
CONTACT:Jo Lockhart
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