Visualizing and controlling the cell cycle machinery in single cells

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• Dr Joerg Mansfeld
• Monday 04 December 2017, 15:00-16:00
• Lecture Theatre, Department of Pharmacology, Tennis Court Road.

If you have a question about this talk, please contact Dr. Cath Lindon.

Cell cycle kinetics are crucial to cell fate decisions. Although live imaging has provided extensive insights into this relationship at the single-cell level, the limited number of fluorescent markers that can be used in a single experiment has hindered efforts to link the dynamics of individual proteins responsible for decision making directly to cell cycle progression. I will present an all-in-one cell cycle reporter that allows simultaneous analysis of cell cycle progression, including the transition into quiescence, and the dynamics of individual fate determinants. Focusing on cyclin D1 as a major cell cycle regulator, we present evidence that a crucial cell cycle function of cyclin D1 is to prevent the transition into quiescence and identify a unique cyclin D1 protein kinetics that are indicative of very short G1 phases. In addition, to allow manipulating the levels of endogenous proteins at will, we introduce a novel approach to render already existing GFP (trap) lines amenable to auxin-dependent degradation by simple ectopic expression of a degradation cassette using human cells and zebrafish as a proofs of principle.

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• Lecture Theatre, Department of Pharmacology, Tennis Court Road

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