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Genome-wide RNAi and HCS Microscopy approaches to dissect membrane traffic pathways

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If you have a question about this talk, please contact Alison Read .

In cells molecules are transported between membrane-bounded compartments through the process of membrane traffic. Due to the exquisite morphology and distribution of organelles in mammalian cells, imaging approaches are highly suited to the study of these transport pathways. Using a combination of genome-wide RNA interference and automated high content screening microscopy we have systematically analysed and quantified the relative contribution of all human genes to the process of secretory transport. Our approach also provides information on the organisation of membranes in the secretory pathway, the subcellular localisation of novel players, and reveals new networks of proteins associated with the regulation of membrane traffic function.

This talk is part of the Genetics Seminar Series series.

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Little Hall Lecture Theatre, Sidgwick Site MR12 ***nonstandard room and time*** Herchel Smith Building Seminar Room Ground Floor, IPH

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