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How do chromatin remodelling enzymes reshape chromatin?

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Any biological process involving the genome – be it transcription, replication or repair – requires the mechanical remodelling of histone-DNA interactions. This remodelling is achieved by sophisticated ATP -dependent DNA translocase enzymes. We have set out to determine the mechanism by which one prominent remodeller that is conserved across multicellular organisms – CHD4 – can move histones relative to DNA . I will describe our results to date and the mechanistic and functional insights that they have provided.

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