Micro Electron Diffraction in a Cryo Electron Microscope: A powerful tool for protein and small molecule structure determination

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• Dr Abhay Kotecha, Materials and Structural Analysis Division, Thermo Fisher Scientific, Eindhoven, The Netherlands
• Wednesday 27 February 2019, 11:00-12:00
• Department of Biochemistry, Sanger Building Jean Thomas Lecture Theatre.

If you have a question about this talk, please contact Andrzej Szewczak-Harris.

In this talk I will show how we can use electron cryo-microscope (CryoEM) to collect electron diffraction from micro- and nano-crystals yielding high-resolution protein structures in a quick and easy way with MicroED method. MicroED can bypass the hurdles of crystal size and radiation damage allowing for the use of extremely small crystals for protein and small molecule structure determination at atomic resolution. MicroED also gives researchers the opportunity to characterize newly synthesized compounds “on the fly,” meaning they can gather important details about a sample throughout its creation, potentially guiding the chemical development process. This is an exciting time for electron crystallographers and those who would like to use micro and nanocrystals. There is much work to be done improving and optimizing this technique, however, we are currently working on testing and automating the workflow to make microED fast and easy, and easily accessible to all users.

This talk is part of the Seminars at the Department of Biochemistry series.

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