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SUMMARY:Crossover frequency mapping across resistance gene clusters in Ara
 bidopsis thaliana and Triticum aestivum  - Carsten Reinhard
DTSTART:20130516T150000Z
DTEND:20130516T153000Z
UID:TALK44290@talks.cam.ac.uk
CONTACT:Megan Cooper
DESCRIPTION:The distribution of meiotic crossover events is not random alo
 ng chromosomes\, but the mechanisms controlling their positioning are as y
 et poorly understood. Taking a comparative approach between Arabidopsis th
 aliana and Triticum aestivum\, the aim of my project is to establish how c
 rossover frequency across regions rich in disease resistance genes compare
 s to the genome average\, and how recombination events contribute to resis
 tance gene supercluster evolution. \nI characterised a number of T-DNA and
  Ds insertion lines in A. thaliana to set up a double marker recombinant s
 election system with view to single nucleotide polymorphism (SNP) assisted
  mapping of crossover events across resistance gene superclusters on chrom
 osomes 1 and 5. While this project is still work in progress\, I can prese
 nt a crossover frequency map with a fineness of 50kb for the investigated 
 5Mb region on chromosome 5. I also discovered a number of recombination ho
 tspots\, including a 10kb interval of 30cM/Mb containing a tandemly repeat
 ed resistance gene\, which I am currently characterising further. In addit
 ion\, I employed a fluorescent pollen reporter line and fluorescence-assoc
 iated cell sorting to assess if the recombination rate across the resistan
 ce gene supercluster on chromosome 5 differs between a range of 20 A. thal
 iana ecotypes and correlates with the degree of sequence polymorphism. \nI
 n T. aestivum\, I produced and utilised a doubled female haploid populatio
 n of 1\,000 individuals derived from a cross between two divergent varieti
 es (Chinese Spring and Renan) for SNP genotyping to map crossover events a
 cross an interval on the best characterised chromosome 3B rich in resistan
 ce genes. I can present a preliminary crossover map for this region\, whic
 h should in the longer term provide insights into the patterns of meiotic 
 recombination of importance for agricultural breeding.\n
LOCATION:Biffen Lecture Theater\, Department of Genetics
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