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SUMMARY:Plasma membrane electron transport in the green alga Chlamydomonas
   reinhardtii - Xander Anderson
DTSTART:20110526T153000Z
DTEND:20110526T160000Z
UID:TALK30689@talks.cam.ac.uk
CONTACT:Suzy Stoodley
DESCRIPTION:Biophotovoltaic (BPV) bioenergy devices capture electrons extr
 uded by single-celled algae and convert these into a power current. Mechan
 isms of electron extrusion in algae are unclear\, though the plasma membra
 ne (PM) is a putative barrier. Chlamydomonas reinhardtii is being used to 
 investigate this process. C.reinhardtii harbours two key PM electron trans
 port candidates: iron reductases and NADPH oxidases (NOX). NOX proteins ge
 nerate superoxide anion (O2.-) by transporting cytosolic electrons to apop
 lastic oxygen. An assay was developed to measure O2.- production. O2.- pro
 duction by C.reinhardtii appears to be sensitive to known NOX inhibitors a
 nd is light-dependent. In addition\, the biological function of the C.rein
 hardtii NOX is being elucidated using RT-PCR. Bioinformatics analyses\, NO
 X assay development\, and RT-PCR will be discussed\, with an emphasis on u
 sing these to investigate PM electron transport.
LOCATION:Department of Plant Sciences\, Large Lecture Theatre
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