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CATEGORIES:Biophysical Colloquia
SUMMARY:Host matrix-pathogen interactions and redox sensin
 g\; tandem beta-zippers and &quot\;inside-out&quot
 \; protein-protein recognition. -  Dr. Jennifer Po
 tts  University of York
DTSTART;TZID=Europe/London:20101103T103000
DTEND;TZID=Europe/London:20101103T113000
UID:TALK27029AThttp://talks.cam.ac.uk
URL:http://talks.cam.ac.uk/talk/index/27029
DESCRIPTION:The interaction between Staphylococcus aureus and 
 Streptococcus pyogenes with the human plasma and e
 xtracellular matrix protein fibronectin triggers b
 acterial invasion of host cells. This process has 
 been proposed to aid haematogenous dissemination o
 f infection and evasion of the host immune system.
  We have used a range of biophysical techniques to
  define the binding sites in the host and bacteria
 l proteins and the structure of the complex that f
 orms. In a highly unusual mechanism of protein-pro
 tein recognition\, the bacterial peptide undergoes
  a transition from an intrinsically disordered sta
 te\, to an extended anti-parallel β-strand along t
 he triple-stranded β-sheets of up to five sequenti
 al fibronectin modules (1-3). A dissection of the 
 S. aureus and S. pyogenes fibronectin-binding prot
 eins\, based on the tandem β-zipper and confirmed 
 using isothermal titration calorimetry\, has ident
 ified multiple high affinity binding sites in both
  proteins (4\,5). \nIn a separate project\, we hav
 e been investigating a redox-sensing anti-sigma fa
 ctor (RsrA) from Streptomyces coelicolor. We have 
 determined the solution structures of the reduced 
 and oxidized forms of RsrA. Comparison with the st
 ructures of homologous anti-sigma factors in compl
 ex with their cognate sigma factors suggests anoth
 er unusual mechanism of protein-protein recognitio
 n.\n\n1.	Schwarz-Linek et al. (2003) Nature 423\, 
 177-181\n2.	Bingham et al. (2008) Proc. Natl Acad.
  Sci. USA 105\, 12254-12258\n3.	Atkin et al.\, (20
 10) J. Biol. Chem. (in press)\n4.	Schwarz-Linek et
  al. (2004) J. Biol. Chem. 279\, 39017-39025\n5.	M
 eenan et al. (2007) J. Biol. Chem. 282\, 25893-259
 02\n
LOCATION:Unilever Lecture Theatre\,  Department of Chemistr
 y
CONTACT:Dr Alfonso De Simone
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