RNA-based regulation mechanism during transcription and translation

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• Professor Harald Schwalbe, Goethe Universität, Frankfurt
• Wednesday 15 March 2017, 10:30-11:30
• Todd-Hamied Room, Department of Chemistry, Lensfield Road, Cambridge.

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Riboswitches are RNA -based regulation elements that modulate gene expression at the level of transcription or translation. By binding of small molecular weight metabolites, they can turn gene expression OFF or ON. In the talk, biophysical studies and simulations will be presented to delineate the molecular mechanism of riboswitches. For translational riboswitches, it is shown that three state mechanism are essential to maintain riboswitch regulation over a broad temperature range (Reining et al., 2013). For transcriptional riboswitches, the kinetic requirements to couple mRNA synthesis, RNA refolding and ligand binding are delineated (Helmling et al., 2017).

A. Reining, S. Nozinovic, K. Schlepckow, F. Buhr, B. Fürtig, H. Schwalbe (2013) Three-state mechanism couples ligand and temperature sensing in riboswitches. Nature 499, 355-359.

C. Helmling, A. Wacker, M.T. Wolfinger, I.L. Hofacker, M. Hengesbach, B. Fürtig, H. Schwalbe (2017) NMR structural profiling of transcriptional intermediates reveals riboswitch regulation by metastable RNA conformations. J. Am. Chem. Soc.139, 2647-2656.

This talk is part of the Biophysical Seminar Series 2016/17 series.

This talk is included in these lists:

• Biophysical Seminar Series 2016/17
• Todd-Hamied Room, Department of Chemistry, Lensfield Road, Cambridge

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